Training Effects on ROS Production Determined by Electron Paramagnetic Resonance in Master Swimmers. (22nd March 2015)

• Record Type: Journal Article
• Authors: Mrakic-Sposta, Simona; Gussoni, Maristella; Porcelli, Simone; Pugliese, Lorenzo; Pavei, Gaspare; Bellistri, Giuseppe; Montorsi, Michela; Tacchini, Philippe; Vezzoli, Alessandra
• Other Names: Radak Zsolt Academic Editor.
• Is Part Of: Oxidative medicine and cellular longevity. Volume 2015(2015)
• Publication Date: 2015-03-22
• Subjects: Oxidative stress -- Periodicals; Cells -- Aging -- Periodicals; Cells -- Aging; Oxidative stress; Oxidative Stress -- Periodicals; Cell Aging -- Periodicals; Periodicals; 611.0181
• DOI: 10.1155/2015/804794
• Languages: English
• ISSNs: 1942-0900

Abstract

Abstract : Acute exercise induces an increase in Reactive Oxygen Species (ROS) production dependent on exercise intensity with highest ROS amount generated by strenuous exercise. However, chronic repetition of exercise, that is, exercise training, may reduce exercise-induced oxidative stress. Aim of this study was to evaluate the effects of 6-weeks high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on ROS production and antioxidant capacity in sixteen master swimmers. Time course changes of ROS generation were assessed by Electron Paramagnetic Resonance in capillary blood by a microinvasive approach. An incremental arm-ergometer exercise (IE) until exhaustion was carried out at both before (PRE) and after (POST) training (Trg) period. A significant (P < 0.01 ) increase of ROS production from REST to the END of IE in PRE Trg (2.82 ± 0.66 versus3.28 ± 0.66 µ mol·min −1 ) was observed. HIDT increased peak oxygen consumption (36.1 ± 4.3 versus40.6 ± 5.7 mL·kg −1 ·min −1 PRE and POST Trg, resp.) and the antioxidant capacity (+13%) while it significantly decreased the ROS production both at REST (−20%) and after IE (−25%). The observed link between ROS production, adaptive antioxidant defense mechanisms, and peak oxygen consumption provides new insight into the correlation between ROS response pathways and muscle metabolic function.