Histamine mediates the pro-inflammatory effect of latex of Calotropis procera in rats. Issue 5 (2003)

• Record Type: Journal Article
• Authors: Shivkar Shivkar, Yatin M. Yatin M.; Kumar Kumar, Vijay L. Vijay L.
• Is Part Of: Mediators of inflammation. Volume 12:Issue 5(2003)
• Page Start: 299
• Page End: 302
• Publication Date: 2003
• Subjects: Calotropis procera -- Carrageenan -- Histamine -- Inflammation -- Anti-inflammatory drugs; Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1080/096293503310001619708
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Introduction: Calotropis procera is known to produce contact dermatitis and the latex of this plant produces intense inflammation when injected locally. However, the precise mode of its pro-inflammatory effect is not known. In present study we have pharmacologically characterized the inflammation induced by latex of C. procera in a rat paw edema model and determined the role of histamine in latex-induced inflammation. Methods: Inflammation was induced in the hind paw of rats by injecting different doses of dried latex (DL) of C. procera . The inhibitory effect of phenylbutazone, dexamethasone, celecoxib, cyproheptadine, chlorpheniramine and compound 48/80 on edema volume was evaluated and compared with that against carrageenan. The histamine content of DL was measured fluorometrically. Results: DL produced dose-dependent inflammation of the rat paw. Cyproheptadine and chlorpheniramine effectively inhibited DL-induced inflammation (90%;P < 0.01 ), while anti-inflammatory drugs phenylbutazone, dexamethasone and celecoxib were more effective against carrageenan-induced inflammation. Depletion of mast cell histamine by compound 48/80 produced a significant decrease in DL-induced inflammation as compared with carrageenan (50% versus 25%). DL was also found to contain about 6 μg/g of histamine. Conclusions: Thus, our study shows that the biogenic amines play a significant role in C. procera latex-induced inflammation and antihistaminic drugs could be effectively used to inhibit inflammatory response elicited by exposure to latex.