Flutolanil affects circadian rhythm in zebrafish (Danio rerio) by disrupting the positive regulators. (August 2019)

• Record Type: Journal Article
• Authors: Yang, Yang; Dong, Fengshou; Liu, Xingang; Xu, Jun; Wu, Xiaohu; Zheng, Yongquan
• Is Part Of: Chemosphere. Volume 228(2019)
• Page Start: 649
• Page End: 655
• Publication Date: 2019-08
• Subjects: Zebrafish -- Flutolanil -- Circadian rhythm -- Cry -- Clock; MT melatonin -- GH growth hormone -- DA dopamine -- UGT1 UDP glucuronosyltransferase 1 -- MAO monoamine oxidase -- TH tamm-horsfall -- DBH dopamine beta-hydroxylase protein -- BMAL brain and muscle ARNT-like -- AANAT arylakylamine N-acetyltransferase -- PER Period -- CRY cryptochrome -- IGF insulin-like growth factor 1 -- BMP bone morphogenetic protein -- LOX lysine oxidase -- CYP cytochrome P450 proteins -- HE1A hatching enzyme 1a -- DMRT1 double-sexandmab-3 related transcription factor1 -- HSD hydroxysteroid dehydrogenase; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.04.207
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Circadian rhythms are fundamental to behavior and physiology of organisms. Flutolanil as a fungicide is toxic to zebrafish embryos. The aims of this study were to determine whether flutolanil would influence circadian rhythms of zebrafish and the mechanism involved. Zebrafish embryos were exposed to flutolanil (0, 0.125, 0.5 and 2 mg/L) for 4 days. Here we report that flutolanil increased the melatonin levels of zebrafish. The mRNA levels of genes related to circadian rhythms were significantly altered. The clock level was significantly increased, but the content of cry1 showed no apparent changes. Moreover, our findings that the level of GH was significantly decreased were consistent with the abnormal development of zebrafish embryos. The expression levels of genes related to development, behavior and reproduction were significantly altered by flutolanil. These results indicate that flutolanil disturbed circadian rhythms of zebrafish primarily by affecting the positive elements, which were at least in partial responsible for abnormal development and behavior of zebrafish. And we speculate that flutolanil is toxic to zebrafish embryos at least in part via dysregulation of circadian rhythms involving clock. Highlights: Flutolanil induced abnormal development and behavior of zebrafish. Flutolanil significantly enhanced the MT contents. Flutolanil significantly up-regulated the clock contents. Flutolanil altered the expression levels of the positive genes.