NDRG1 negatively regulates proliferation and Milk bio-synthesis of bovine epithelial cells via the mTOR signaling pathway. (June 2019)

• Record Type: Journal Article
• Authors: Li, Xueru; Zhang, Pengyuan; Wang, Bingbing; Zhang, Jinjing; Zhang, Yong; Gao, Ming-Qing
• Is Part Of: Research in veterinary science. Volume 124(2019)
• Page Start: 158
• Page End: 165
• Publication Date: 2019-06
• Subjects: Mastitis -- MAC-T -- NDRG1 -- mTOR -- β-Casein -- Proliferation; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2019.03.007
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: The expression of N-myc downstream-regulated gene 1 (NDRG1) was significantly correlated with diverse processes such as cell growth and differentiation, lipid synthesis, stress and immune responses. Here we explored the role of NDRG1 expression in bovine mammary tissue and epithelial cells under an inflammatory condition. Results showed that NDRG1 expression was elevated in bovine mammary tissue with mastitis and mammary epithelial cells treated by heat inactivated Escherichia coli and Staphylococcus aureus compared to normal tissue and untreated cells . Overexpression of NDRG1 significantly inhibited cell proliferation and migration, β-casein secretion, gene expressions of inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-6 and IL-8, and activation of mTOR signal pathway of mammary epithelial cells, and vice versa by NDRG1 knockdown. These findings suggest that NDRG1 has immense potential in the regulation of properties in bovine mammary epithelial cells under an inflammatory condition. Highlights: NDRG1 expression was elevated in bovine mammary tissue and epithelial cells under inflammatory conditions. NDRG1 inhibited gene expressions of inflammatory cytokines of TNF-α, IL-6 and IL-8. NDRG1 negatively regulates proliferation, migration and β-casein secretion of MAC-T cells. NDRG1 functions via the mTOR signaling pathway.