Gamma-Tocotrienol Stimulates the Proliferation, Differentiation, and Mineralization in Osteoblastic MC3T3-E1 Cells. (15th January 2018)

• Record Type: Journal Article
• Authors: Xu, Weili; He, Pan; He, Shenghua; Cui, Pengju; Mi, Yaqing; Yang, Yang; Li, Yang; Zhou, Shaobo
• Other Names: Kang Ji Academic Editor.
• Is Part Of: Journal of chemistry. Volume 2018(2018)
• Publication Date: 2018-01-15
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1155/2018/3805932
• Languages: English
• ISSNs: 2090-9063

Abstract

Abstract : Gamma-tocotrienol, a major component of tocotrienol-rich fraction of palm oil, has been suggested to exhibit bone protective effects in vivo . However, the effects of γ -tocotrienol on osteoblast cells are still unclear. In this study, the effects of γ -tocotrienol on the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cells were investigated. Our results showed that γ -tocotrienol (2–8 μ mol/L) significantly improved the cell proliferation (p < 0 . 05 ), but it did not affect cell cycle progression. γ -Tocotrienol significantly increased alkaline phosphatase (ALP) activity (p < 0 . 05 ), secretion levels of osteocalcin (OC) and osteonectin (ON), and mRNA levels of collagen type I (Col I) of MC3T3-E1 cells. Meanwhile, we found that γ -tocotrienol is promoted in differentiation MC3T3-E1 cells by upregulation of the expression of Runx2 protein. Moreover, the number of bone nodules increased over 2.5-fold in cells treated with γ -tocotrienol (2–8 μ mol/L) for 24 d compared to control group. These results indicated that γ -tocotrienol at low dose levels, especially 4 μ mol/L, could markedly enhance the osteoblastic function by increasing the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells. Moreover, our data also indicated that Runx2 protein may be involved in these effects. Further studies are needed to determine the potential of γ -tocotrienol as an antiosteoporotic agent.