The effect of electroconvulsive seizure on survival, neuronal differentiation, and expression of the maturation marker in the adult mouse hippocampus. Issue 4 (2nd April 2019)

• Record Type: Journal Article
• Authors: Ueno, Miyuki; Sugimoto, Mami; Ohtsubo, Keisuke; Sakai, Naoto; Endo, Akane; Shikano, Kisako; Imoto, Yuki; Segi‐Nishida, Eri
• Is Part Of: Journal of neurochemistry. Volume 149:Issue 4(2019)
• Page Start: 488
• Page End: 498
• Publication Date: 2019-04-02
• Subjects: antidepressant -- electroconvulsive seizure -- granule cell -- hippocampus -- maturation -- neurogenesis; Neurochemistry -- Periodicals; 616.8042
• DOI: 10.1111/jnc.14691
• Languages: English
• ISSNs: 0022-3042

Abstract

Abstract: Electroconvulsive seizure (ECS), a model of electroconvulsive therapy in rodents, strongly increases neurogenesis in the adult hippocampus. Neurogenesis is a multi‐step process that spans proliferation, survival, neuronal differentiation, and functional maturation. Our previous study demonstrated that ECS stimulates the proliferation of neural stem‐like cells. However, the contribution of ECS to survival, neuronal differentiation, and maturation in newborn cells remains unknown. To evaluate the effect of ECS on these processes, we labeled newborn cells with bromodeoxyuridine (BrdU) before ECS treatment to determine the cell age and examined the survival rate and expression of cellular markers in the BrdU‐labeled cells. Our results revealed that exposure to ECS (11 repetitions) during the differentiation phase significantly increased survival and promoted neuronal differentiation of newborn cells in the dentate gyrus. Four of ECS repetitions during the early differentiation phase were sufficient to promote dendritic outgrowth in immature neurons and enhance the expression of the immature neuronal marker, calretinin, in newborn cells. In contrast, exposure to ECS (11 repetitions) during the late maturation phase significantly suppressed the expression of the mature neuronal marker, calbindin, in newborn neurons. These results demonstrate that ECS during the differentiation phase promoted survival and neuronal differentiation and, in contrast, suppressed mature marker expression during the late maturation phase, suggesting that ECS has multiple effects on the different stages of adult neurogenesis. Abstract : Electroconvulsive seizure (ECS), a model of electroconvulsive therapy in rodents, strongly increases neurogenesis in the adult hippocampus. However, the contribution of ECS to survival, neuronal differentiation, and maturation remains unknown. Our results revealed that exposure to ECS increased survival and neuronal differentiation of newborn cells in the dentate gyrus but suppressed the expression of the mature neuronal marker calbindin. These results suggest that ECS has multiple effects on the different stages of adult neurogenesis.