Dentinal tubule penetration of endodontic sealers after nonthermal plasma treatment: A confocal laser scanning microscopy study. Issue 6 (27th February 2019)

• Record Type: Journal Article
• Authors: Gunes, Betul; Yeter, Kubra Y.; Terlemez, Arslan; Seker, Basak; Altay, Yasin
• Is Part Of: Microscopy research and technique. Volume 82:Issue 6(2019)
• Page Start: 903
• Page End: 908
• Publication Date: 2019-02-27
• Subjects: confocal laser scanning microscopy -- dentinal tubule penetration -- Endosequence BC -- nonthermal plasma treatment; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23237
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: One of the factors affecting the success of endodontic treatment is to fill the root canal system hermetically. The aim of this in vitro study was to evaluate the effect of nonthermal plasma (NP) on dentinal tubule penetration of root canal sealers using confocal laser scanning microscopy. Forty mandibular premolar teeth were selected and the root canals were prepared with large‐Waveone‐Gold rotary‐files. Specimens were divided into four experimental groups according to sealer and NP treatment ( n = 10). G1: AH‐Plus (AH) G2: nonthermal plasma application + AH‐Plus(AH‐P) G3: Endosequence‐BC(BC) G4: nonthermal plasma application + Endosequence‐BC(BC‐P). Cold lateral‐condensation technique was used for the obturation of root canals. The roots were sectioned horizontally and the sections were examined under confocal laser scanning microscopy. The maximum tubule penetration and percentage of penetration values were obtained from the microscopy images and were statistically analyzed with repeated measurements‐ANOVA and the Tukey (HSD) test ( p < 0.05). The percentages of dentinal tubule penetration of the groups were not statistically different. The maximum tubule penetration of the AH‐P was statistically lower than that of the BC‐P ( p < 0.05). Plasma application had no affect on the percentage of dentinal tubule penetration. Under the conditions of this in vitro Endosequence‐BC sealer showed higher maximum tubule penetration values than AH‐Plus after NP treatment. Percentage of dentinal tubule penetration values of experimental groups was similar. Abstract : This is an example image obtained using CLS microscopy. Dentinal tubule penetration values were measured using these images. According to results of this study evaluating the dentinal tubule penetration using confocal laser scanning microscopy, The percentage of dentinal tubule penetration was evaluated and there was no statistically significant difference between the experimental groups. Maximum tubule penetration value of Endosequence BC was higher than AH Plus after nonthermal plasma treatment. Maximum tubule penetration values were lower in apical sections than coronal sections for each experimental group.