Extraction of Saponin from Camellia oleifera Abel Cake by a Combination Method of Alkali Solution and Acid Isolation. (22nd February 2016)

• Record Type: Journal Article
• Authors: Liu, Yongjun; Li, Zhifeng; Xu, Hongbo; Han, Yuanyuan
• Other Names: Moldes Ana Academic Editor.
• Is Part Of: Journal of chemistry. Volume 2016(2016)
• Publication Date: 2016-02-22
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1155/2016/6903524
• Languages: English
• ISSNs: 2090-9063

Abstract

Abstract : Saponin 15%~20% content in the seed cake of Camellia oleifera Abel, from which Camellia oil is squeezed, is a natural nonionic surface active agent and is extensively applied to emulsification, humectation, foaming, medicine, pesticide, and so on. In this paper, the extraction process of saponin was researched through a combining method of alkali solution and acid isolation. A quantitative method for saponin was established by ultraviolet spectrophotometer. The influence of extraction factors was investigated by a single-factor test and a response surface methodology. The results indicated that the optimal extraction conditions of saponin were extraction temperature 68°C, alkali solution pH 9.1, acid isolation pH 4.1, and liquid-solid ratio 15.9 : 1. The extraction rate of saponin was 76.12% at the optimal extraction conditions.