High-throughput electrical position detection of single flowing particles/cells with non-spherical shape. Issue 10 (18th April 2019)

• Record Type: Journal Article
• Authors: Reale, Riccardo; De Ninno, Adele; Businaro, Luca; Bisegna, Paolo; Caselli, Federica
• Is Part Of: Lab on a chip. Volume 19:Issue 10(2019)
• Page Start: 1818
• Page End: 1827
• Publication Date: 2019-04-18
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c9lc00071b
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A novel all-electrical approach for cross-sectional position detection of flowing particles and cells. Abstract : We present an innovative impedance cytometer for the measurement of the cross-sectional position of single particles or cells flowing in a microchannel. As predicted by numerical simulations and experimentally validated, the proposed approach is applicable to particles/cells with either spherical or non-spherical shape. In particular, the optics-free high-throughput position detection of individual flowing red blood cells (RBCs) is demonstrated and applied to monitor RBCs hydrodynamic focusing under different sheath flow conditions. Moreover, the device provides multiparametric information useful for lab-on-a-chip applications, including particle inter-arrival times and velocity profile, as well as RBCs mean corpuscular volume, distribution width and electrical opacity.