Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer. Issue 39 (26th April 2019)

• Record Type: Journal Article
• Authors: Ali, Aysha; Bullen, Gemma A.; Cross, Benjamin; Dafforn, Timothy R.; Little, Haydn A.; Manchester, Jack; Peacock, Anna F. A.; Tucker, James H. R.
• Is Part Of: Chemical communications. Volume 55:Issue 39(2019)
• Page Start: 5627
• Page End: 5630
• Publication Date: 2019-04-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc01540j
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The application of light distorts the structure of an anthracene-functionalized DNA aptamer, preventing its inhibitive binding to the clotting enzyme thrombin, resulting in photoswitched catalysis. Abstract : The reversible photocontrol of an enzyme governing blood coagulation is demonstrated. The thrombin binding aptamer (TBA ), was rendered photochromic by modification with two anthracene groups. Light-triggered anthracene photodimerisation distorts its structure, inhibiting binding of the enzyme thrombin, which in turn triggers catalysis and the resulting clotting process.