Modulation of ICT and PET processes in boranil derivatives: a ratiometric fluorescent probe for imaging of cysteine. Issue 19 (7th May 2019)

• Record Type: Journal Article
• Authors: Zhu, Dongjian; Yan, Xiaowei; Ren, Aishan; Cai, Wen; Duan, Zhenhua; Luo, Yanghe
• Is Part Of: Analytical methods. Volume 11:Issue 19(2019)
• Page Start: 2579
• Page End: 2584
• Publication Date: 2019-05-07
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c9ay00235a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : 1 is the first ratiometric fluorescent probe for imaging of intracellular Cys based on boranil derivatives modulated by ICT and PET processes with fast response, high sensitivity, excellent selectivity. Abstract : A novel ratiometric fluorescent probe1 based on boranil derivatives modulated by ICT and PET processes was designed and synthesized for the first time. Upon addition of Cys, the emission peak of1 changed from 461 nm to 474 nm, accompanied by a fluorescence color change from dark blue to blue under illumination with a 365 nm UV lamp.1 exhibited a fast response (within 9 min), high sensitivity (detection limit of 95.1 nM) and excellent selectivity toward Cys over Hcy and GSH owing to a specific conjugate addition–cyclization reaction between Cys and the acrylate moiety. Furthermore, 1 was successfully implemented for monitoring Cys in living cells.