Chiral separation of d/l-arginine with whole cells through an engineered FhuA nanochannel. Issue 38 (27th March 2019)

• Record Type: Journal Article
• Authors: Anand, Deepak; Dhoke, Gaurao V.; Gehrmann, Julia; Garakani, Tayebeh M.; Davari, Mehdi D.; Bocola, Marco; Zhu, Leilei; Schwaneberg, Ulrich
• Is Part Of: Chemical communications. Volume 55:Issue 38(2019)
• Page Start: 5431
• Page End: 5434
• Publication Date: 2019-03-27
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc00154a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Development of a novel whole cell system for chiral separation of arginine enantiomers through an engineered outer membrane channel protein ferric hydroxamate uptake protein A (FhuA). Abstract : Downstream processing to obtain enantiopure compounds from a racemic mixture relies mainly on crystallization. Natural transporters can specifically translocate enantiomers through membranes. Here a β-barrel transmembrane protein FhuA is re-engineered into a chiral channel protein (FhuAF4) to resolve racemic mixtures ofd -/l -arginine. The engineered FhuAF4 variant exhibits an enantioselectivity ( E -value) of 1.92 and an enantiomeric excess percentage (ee%) of 23.91 at 52.39% conversion. OmniChange mutant libraries at the computationally identified "filter-regions" likely help to identify FhuA variants for enantiomeric separation of other compounds.