A circular RNA circ_0000115 in response to graphene oxide in nematodes. Issue 24 (3rd May 2019)

• Record Type: Journal Article
• Authors: Shi, Lifang; Jia, Xiaohuan; Guo, Tiantian; Cheng, Lu; Han, Xiaoxiao; Wu, Qiuli; Wang, Dayong
• Is Part Of: RSC advances. Volume 9:Issue 24(2019)
• Page Start: 13722
• Page End: 13735
• Publication Date: 2019-05-03
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c9ra00997c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Circular RNAs (circRNAs) play important roles in regulating various biological processes; however, their roles in regulating the toxicity of engineered nanomaterials (ENMs) are still unclear. Abstract : Circular RNAs (circRNAs) play important roles in regulating various biological processes; however, their roles in regulating the toxicity of engineered nanomaterials (ENMs) are still unclear. Based on Illumina HiSeq2500 sequencing, we here identified 43 dysregulated circRNAs in graphene oxide (GO) (1 mg L −1 ) exposed nematodes. Five of these candidate circRNAs could be further dysregulated by GO exposure in the range of μg L −1 . Using the RNA interference (RNAi) technique, we found that the alteration in expressions of circ_0000115, circ_0000247, and circ_0000665 mediated a protective response to GO exposure; however, the alteration in expressions of circ_0000201 and circ_0000308 mediated the toxicity induction of GO. In nematodes, the circ_0000115 acted in certain tissues (intestine and neurons) to regulate GO toxicity. Moreover, an intermediate filament protein IFC-2 required for intestinal development was identified as a target of circ_0000115 in regulating the GO toxicity. In the intestine, intestinal IFC-2 acted further upstream of FOXO transcriptional factor DAF-16 in the insulin signaling pathway to regulate the GO toxicity. Therefore, intestinal circ_0000115 in the signaling cascade of circ_0000115 -IFC-2-DAF-16 regulates the GO toxicity by modulating the function of IFC-2.