Automatic screening of antioxidants based on the evaluation of kinetics of suppression of chemiluminescence in a luminol–hydrogen peroxide system using a sequential injection analysis setup with a flow-batch detection cell. Issue 19 (26th April 2019)

• Record Type: Journal Article
• Authors: Chládková, Gabriela; Kunovská, Klára; Chocholouš, Petr; Polášek, Miroslav; Sklenářová, Hana
• Is Part Of: Analytical methods. Volume 11:Issue 19(2019)
• Page Start: 2531
• Page End: 2536
• Publication Date: 2019-04-26
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c9ay00160c
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Real time monitoring of kinetic profiles of antioxidant activity of phenolic substances. Abstract : A sequential injection analysis system with sensitive chemiluminescence detection using a flow-batch detection cell was developed for the real time monitoring of kinetic profiles of antioxidant activity of phenolic substances in a luminol–Fe(iii )–hydrogen peroxide system during a 60 s stop–flow period. Selected substances including coumaric acid, caffeic acid, hesperidin, ascorbic acid, resveratrol, rutin, chlorogenic acid, quercitrin, epicatechin, phloridzin, phloretin, and Trolox as a reference standard were tested with respect to different chemiluminescence signal kinetics. The evaluation of antioxidant activity was expressed as the quenching efficacy index Q . Our method has a wide working concentration range of 4–250 μmol L −1 for Trolox antioxidant activity. The real time kinetic profiles based on the chemiluminescence quenching half-life of both quick and slow antioxidants were also determined. A sample throughput of 30 h −1 was demonstrated. The antioxidant activity of apple and black elder fruit extracts was evaluated.