Droplet-based single cell RNAseq tools: a practical guide. Issue 10 (18th April 2019)

• Record Type: Journal Article
• Authors: Salomon, Robert; Kaczorowski, Dominik; Valdes-Mora, Fatima; Nordon, Robert E.; Neild, Adrian; Farbehi, Nona; Bartonicek, Nenad; Gallego-Ortega, David
• Is Part Of: Lab on a chip. Volume 19:Issue 10(2019)
• Page Start: 1706
• Page End: 1727
• Publication Date: 2019-04-18
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c8lc01239c
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A step-by-step guide for droplet-based single cell RNAseq experiments, practical considerations and technical notes. Abstract : Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.