Evaluation of Multitudinous Potentials of Photosynthetic Microalga, Neochloris aquatica RDS02 Derived Silver Nanoparticles. Issue 2 (3rd April 2019)

• Record Type: Journal Article
• Authors: Tamil Selvan, Silambarasan; Balasubramani, Govindasamy; Narayanasamy, Selvaraju; Ramamurthy, Dhandapani
• Is Part Of: Smart science. Volume 7:Issue 2(2019)
• Page Start: 116
• Page End: 129
• Publication Date: 2019-04-03
• Subjects: Silver nanoparticles -- Neochloris aquatica RDS02 -- anticancer -- antibacterial -- antiviral
• DOI: 10.1080/23080477.2018.1491743
• Languages: English
• ISSNs: 2308-0477

Abstract

Abstract: The present study pertains to the silver nanoparticles (Ag NPs) synthesis from cell free supernatant of freshwater microalga, Neochloris aquatica RDS02 (KJ700475.1). The synthesized Ag NPs was confirmed with the characteristic features viz., UV–vis Spec: the surface plasmon resonance at 420 nm; XRD: the h k l values with 4 prominent peaks and the average size of NPs (~20 nm); FTIR: functional group involved in bio-reduction; HRTEM: well distributed spherical morphology with size ranged from 15 to 20 nm. The microalga ( N. aquatica ) mediated Ag NPs possesses enhanced antibacterial activity on Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis and Staphylococcus aureus . The anti-proliferative activity on HeLa cell line by MTT and the antiviral activity on Herpes simplex viruses (HSV1 and HSV2) by plaque assays were evaluated using Ag NPs. The Ag NPs caused morphological changes, cytoplasmic content leakage, and membrane permeabilization in a dose-dependent manner. The IC50 values (μg mL −1 ) of Ag NPs were found to be 39.5 for HeLa and 100 for HEp2 viral cells. Hence, the Ag NPs would be considered as a promising biological agent and emerge as an alternative for drug development to combat the problem over the existing pharmaceuticals. Abstract :