One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal. (28th June 2018)

• Record Type: Journal Article
• Authors: Machado, Juliana R.; Severo, Emanuelle E.; de Oliveira, Janaina M. G.; Ores, Joana da C.; Brandelli, Adriano; Kalil, Susana J.
• Other Names: Feke Donald L. Academic Editor.
• Is Part Of: International journal of chemical engineering. Volume 2018(2018)
• Publication Date: 2018-06-28
• Subjects: Chemical engineering -- Periodicals; Chemical engineering; Electronic journals; Periodicals; 660
• DOI: 10.1155/2018/6729490
• Languages: English
• ISSNs: 1687-806X

Abstract

Abstract : A purification technique to obtain keratinolytic proteases produced by Bacillus sp. P45 in a medium containing chicken feather meal as substrate is presented. The experiments were carried out in a dead-end ultrafiltration unit, and the influence of the membrane cutoff, pH of enzymatic extract, and operating pressure on the purification of keratinase were studied. The one-step ultrafiltration process with the membrane molecular mass cutoff of 10 kDa at pH 8.0 and operating pressure of 0.147 MPa showed an enzyme recovery of 87.8% and a 4.1-fold purification factor. It is showed that ultrafiltration could be potentially used in the purification of keratinases.