Β-Glucan Oligosaccharide Enhances CD8+ T Cells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen. (6th May 2010) - Wang, Jing
Abstract : DNA vaccination can induce specificCD 8 + T cell immune response, but the response level is low in large mammals and human beings. Coadministration of an adjuvant can optimize protective immunity elicited by a DNA vaccine. In this study, we investigated the effect of a synthetic glucohexaose (β -glu6), an analogue of Lentinan basic unit, on specificCD 8 + T cell response induced by a DNA vaccine encoding HBcAg (pB144) in mice. We found thatβ -glu6 promoted the recruitment and maturation of dendritic cells, enhanced the activation ofCD 8 + andCD 4 + T cells and increased the number of specificCD 8 + /IFN-γ + T cells in lymphoid and nonlymphoid tissues in mice immunized by pB144. Immunization with pB144 andβ -glu6 increased the anti-HBc IgG and IgG2a antibody titer. These results demonstrate thatβ -glu6 can enhance the virus-specific CTL and Th1 responses induced by DNA vaccine, suggestingβ -glu6 as a candidate adjuvant in DNA vaccination.