Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant. (12th December 2016)

• Record Type: Journal Article
• Authors: Park, Miseon; Mitchell, Wilfrid J.; Rafii, Fatemeh
• Other Names: Iglewski Barbara H. Academic Editor.
• Is Part Of: International journal of microbiology. Volume 2016(2016)
• Publication Date: 2016-12-12
• Subjects: Microbiology -- Periodicals; Microbiology; Microbiology -- Periodicals; Electronic journals; Periodicals; 579
• DOI: 10.1155/2016/4829716
• Languages: English
• ISSNs: 1687-918X

Abstract

Abstract : Trehalose has been shown to protect bacterial cells from environmental stress. Its uptake and osmoprotective effect in Clostridium perfringens were investigated by comparing wild type C. perfringens ATCC 13124 with a fluoroquinolone- (gatifloxacin-) resistant mutant. In a chemically defined medium, trehalose and sucrose supported the growth of the wild type but not that of the mutant. Microarray data and qRT-PCR showed that putative genes for the phosphorylation and transport of sucrose and trehalose (via phosphoenolpyruvate-dependent phosphotransferase systems, PTS) and some regulatory genes were downregulated in the mutant. The wild type had greater tolerance than the mutant to salts and low pH; trehalose and sucrose further enhanced the osmotolerance of the wild type to NaCl. Expression of the trehalose-specific PTS was lower in the fluoroquinolone-resistant mutant. Protection of C. perfringens from environmental stress could therefore be correlated with the ability to take up trehalose.