High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus. (19th January 2014)

• Record Type: Journal Article
• Authors: Singh, Vineet K.
• Other Names: Iglewski Barbara H. Academic Editor.
• Is Part Of: International journal of microbiology. Volume 2014(2014)
• Publication Date: 2014-01-19
• Subjects: Microbiology -- Periodicals; Microbiology; Microbiology -- Periodicals; Electronic journals; Periodicals; 579
• DOI: 10.1155/2014/615965
• Languages: English
• ISSNs: 1687-918X

Abstract

Abstract : Staphylococcus aureus is a major human and animal pathogen. Autolysins regulate the growth, turnover, cell lysis, biofilm formation, and the pathogenicity of S. aureus . Atl is the major autolysin in S. aureus . The biochemical and structural studies of staphylococcal Atl have been limited due to difficulty in cloning, high level overexpression, and purification of this protein. This study describes successful cloning, high level over-expression, and purification of two forms of fully functional Atl proteins. These pure proteins can be used to study the functional and structural properties of this important protein.