Alsterpaullone, a Cyclin-Dependent Kinase Inhibitor, Mediated Toxicity in HeLa Cells through Apoptosis-Inducing Effect. (12th March 2013)

• Record Type: Journal Article
• Authors: Cui, Chunying; Wang, Yuji; Wang, Yaonan; Zhao, Ming; Peng, Shiqi
• Other Names: Sun Feng Academic Editor.
• Is Part Of: Journal of analytical methods in chemistry. Volume 2013(2013)
• Publication Date: 2013-03-12
• Subjects: Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Technique -- Periodicals; 543.05
• DOI: 10.1155/2013/602091
• Languages: English
• ISSNs: 2090-8865

Abstract

Abstract : Alsterpaullone, a small molecule cyclin-dependent kinase (CDK) inhibitor, regulates the cell cycle progression. Beyond death-inducing properties, we identified the effect of alsterpaullone on cycle procedure and apoptosis of HeLa cell. It was found that alsterpaullone inhibited HeLa cells in a time-dependent (0–72 h) and dose-dependent (0–30 μ M) manner. In the presence of alsterpaullone, HeLa cells were arrested in G2 /M prior to undergoing apoptosis via a mechanism that is involved in the regulation of various antiapoptotic genes, DNA-repair, transcription, and cell cycle progression. Compared to controls, alsterpaullone effectively prevented HeLa cells from entering S-phase. These potential therapeutic efficacies could be correlated with the activation of caspase-3.