Positive Selection of Squalene Synthase in Cucurbitaceae Plants. (9th May 2019)
- Record Type:
- Journal Article
- Title:
- Positive Selection of Squalene Synthase in Cucurbitaceae Plants. (9th May 2019)
- Main Title:
- Positive Selection of Squalene Synthase in Cucurbitaceae Plants
- Authors:
- Qian, Jieying
Liu, Yong
Ma, Chengtong
Chao, Naixia
Chen, Qicong
Zhang, Yangmei
Luo, Yu
Cai, Danzhao
Wu, Yaosheng - Other Names:
- Jakubowski Hieronim Academic Editor.
- Abstract:
- Abstract : Triterpenoid saponins are secondary metabolites synthesized through isoprenoid pathways in plants. Cucurbitaceae represent an important plant family in which many species contain cucurbitacins as secondary metabolites synthesized through isoprenoid and triterpenoid pathways. Squalene synthase ( SQS ) is required for the biosynthesis of isoprenoids, but the forces driving the evolution of SQS remain undetermined. In this study, 10 SQS cDNA sequences cloned from 10 species of Cucurbitaceae and 49 sequences of SQS downloaded from GenBank and UniProt databases were analyzed in a phylogenetic framework to identify the evolutionary forces for functional divergence. Through phylogenetic construction and positive selection analysis, we found that SQS sequences are under positive selection. The sites of positive selection map to functional and transmembrane domains. 180L, 189S, 194S, 196S, 265I, 289P, 389P, 390T, 407S, 408A, 410R, and 414N were identified as sites of positive selection that are important during terpenoid synthesis and map to transmembrane domains. 196S and 407S are phosphorylated and influence SQS catalysis and triterpenoid accumulation. These results reveal that positive selection is an important evolutionary force for SQS in plants. This provides new information into the molecular evolution of SQS within the Cucurbitaceae family.
- Is Part Of:
- International journal of genomics. Volume 2019(2019)
- Journal:
- International journal of genomics
- Issue:
- Volume 2019(2019)
- Issue Display:
- Volume 2019, Issue 2019 (2019)
- Year:
- 2019
- Volume:
- 2019
- Issue:
- 2019
- Issue Sort Value:
- 2019-2019-2019-0000
- Page Start:
- Page End:
- Publication Date:
- 2019-05-09
- Subjects:
- Genomes -- Periodicals
Genomics -- Periodicals
Cytogenetics -- Periodicals
Genomics
Genome
Molecular Biology
Cytogenetics
Genomes
Genomics
Periodicals
572.86 - Journal URLs:
- https://www.hindawi.com/journals/ijg/ ↗
http://www.ncbi.nlm.nih.gov/pmc/journals/2080/ ↗
http://bibpurl.oclc.org/web/52605 ↗
http://search.ebscohost.com/direct.asp?db=a9h&jid=%22G611%22&scope=site ↗ - DOI:
- 10.1155/2019/5913491 ↗
- Languages:
- English
- ISSNs:
- 2314-436X
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD Digital store
- Ingest File:
- 10361.xml