Characterization of Modified Magnetite Nanoparticles for Albumin Immobilization. (22nd May 2014)

• Record Type: Journal Article
• Authors: Bordbar, A. K.; Rastegari, A. A.; Amiri, R.; Ranjbakhsh, E.; Abbasi, M.; Khosropour, A. R.
• Other Names: Canovas Manuel Academic Editor.
• Is Part Of: Biotechnology research international. Volume 2014(2014)
• Publication Date: 2014-05-22
• Subjects: Biotechnology -- Periodicals; Biotechnology; Biotechnology; Periodicals; 660.6
• DOI: 10.1155/2014/705068
• Languages: English
• ISSNs: 2090-3138

Abstract

Abstract : Magnetite Fe3 O4 nanoparticles (NPs) were prepared by chemical coprecipitation method. Silica-coated magnetite NPs were prepared by sol-gel reaction, subsequently coated with 3-aminopropyltriethoxysilane (APTES) via silanization reaction, and then were activated with 2, 4, 6-trichloro-1, 3, 5-triazine (TCT) and covalently immobilized with bovine serum albumin (BSA). The size and structure of the particles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and dynamic light scattering (DLS) techniques. The immobilization was confirmed by Fourier transform infrared spectroscopy (FT-IR). XRD analysis showed that the binding process has not done any phase change to Fe3 O4 . The immobilization time for this process was 4 h and the amount of immobilized BSA for the initial value of 1.05 mg BSA was about 120 mg/gr nanoparticles. Also, the influences of three different buffer solutions and ionic strength on covalent immobilization were evaluated.