The Liver X Receptor in Correlation with Other Nuclear Receptors in Spontaneous and Recurrent Abortions. (15th April 2013)

• Record Type: Journal Article
• Authors: Knabl, Julia; Pestka, Aurelia; Hüttenbrenner, Rebecca; Plösch, Torsten; Ensenauer, Regina; Welbergen, Lena; Hutter, Stefan; Günthner-Biller, Maria; Jeschke, Udo
• Other Names: Fournier Thierry Academic Editor.
• Is Part Of: PPAR research. Volume 2013(2013)
• Publication Date: 2013-04-15
• Subjects: Peroxisomes -- Periodicals; Peroxisomal disorders -- Periodicals; 571.65505
• DOI: 10.1155/2013/575604
• Languages: English
• ISSNs: 1687-4757

Abstract

Abstract : The liver X receptors (LXRs) have been shown to be crucially involved in maternal-fetal cholesterol transport and placentation. The aim of this study was to investigate the expression pattern and frequency of LXR under normal physiological circumstances and in spontaneous abortion and/or recurrent miscarriage. A total of 29 (12 physiologic pregnancies/10 spontaneous abortions/7 recurrent miscarriages) human pregnancies in first trimester were analysed for LXR expression. Expression changes were evaluated by immunohistochemistry for receptor and quantitative RT-PCR (TaqMan) was performed to determine the level of LXR mRNA expression. We also stained for RXR α and PPAR γ as possible heterodimers of LXR. LXR expression was downregulated in the syncytiotrophoblast of spontaneous abortion placentas compared to normal pregnancy. In recurrent miscarriage there was a trend for a downregulation. Decidua showed an even stronger downregulation in both groups. In the syncytiotrophoblast we found a positive correlation for the combination of LXR/PPAR γ in abortions and a negative correlation for LXR/RXR α . In addition, double-immunofluorescence staining showed that LXR as well as RXR α and PPAR γ are expressed by the extravillous trophoblast. Finally, RXR α and LXR showed coexpression in the same extravillous trophoblast cells. To conclude, our data show that LXR expression is decreased in miscarriage.