Children Living near a Sanitary Landfill Have Increased Breath Methane and Methanobrevibacter smithii in Their Intestinal Microbiota. (13th October 2014)

• Record Type: Journal Article
• Authors: Bezerra de Araujo Filho, Humberto; Silva Carmo-Rodrigues, Mirian; Santos Mello, Carolina; Cristina Fonseca Lahoz Melli, Lígia; Tahan, Soraia; Carlos Campos Pignatari, Antonio; Batista de Morais, Mauro
• Other Names: Whitman William B. Academic Editor.
• Is Part Of: Archaea. Volume 2014(2014)
• Publication Date: 2014-10-13
• Subjects: Archaebacteria -- Periodicals; 579.321
• DOI: 10.1155/2014/576249
• Languages: English
• ISSNs: 1472-3646

Abstract

Abstract : This study evaluated the breath CH4 excretion and concentration of M. smithii in intestinal microbiota of schoolchildren from 2 slums. One hundred and eleven children from a slum near a sanitary landfill, 35 children of a slum located away from the sanitary landfill, and 32 children from a high socioeconomic level school were included in the study. Real-time PCR was performed to quantify the M. smithii nifH gene and it was present in the microbiota of all the participating children, with higherP < 0.05 concentrations in those who lived in the slum near the landfill (3.16 × 10 7 CFU/g of feces), comparing with the children from the slum away from the landfill (2.05 × 10 6 CFU/g of feces) and those from the high socioeconomic level group (3.93 × 10 5 CFU/g of feces). The prevalence of children who present breath methane was 53% in the slum near the landfill, 31% in the slum further away from the landfill and, 22% in the high socioeconomic level group. To live near a landfill is associated with higher concentrations of M. smithii in intestinal microbiota, comparing with those who live away from the landfill, regardless of their socioeconomics conditions.