Synthetic Archaeosome Vaccines Containing Triglycosylarchaeols Can Provide Additive and Long-Lasting Immune Responses That Are Enhanced by Archaetidylserine. (30th September 2012)

• Record Type: Journal Article
• Authors: Sprott, G. Dennis; Yeung, Angela; Dicaire, Chantal J.; Yu, Siu H.; Whitfield, Dennis M.
• Other Names: Corcelli Angela Academic Editor.
• Is Part Of: Archaea. Volume 2012(2012)
• Publication Date: 2012-09-30
• Subjects: Archaebacteria -- Periodicals; 579.321
• DOI: 10.1155/2012/513231
• Languages: English
• ISSNs: 1472-3646

Abstract

Abstract : The relation between archaeal lipid structures and their activity as adjuvants may be defined and explored by synthesizing novel head groups covalently linked to archaeol (2, 3-diphytanyl-sn-glycerol). Saturated archaeol, that is suitably stable as a precursor for chemical synthesis, was obtained in high yield from Halobacterium salinarum . Archaeosomes consisting of the various combinations of synthesized lipids, with antigen entrapped, were used to immunize mice and subsequently determine CD8 + and CD4 + -T cell immune responses. Addition of 45 mol% of the glycolipids gentiotriosylarchaeol, mannotriosylarchaeol or maltotriosylarchaeol to an archaetidylglycerophosphate-O-methyl archaeosome, significantly enhanced the CD8 + T cell response to antigen, but diminished the antibody titres in peripheral blood. Archaeosomes consisting of all three triglycosyl archaeols combined with archaetidylglycerophosphate-O-methyl (15/15/15/55 mol%) resulted in approximately additive CD8 + T cell responses and also an antibody response not significantly different from the archaetidylglycerophosphate-O-methyl alone. Synthetic archaetidylserine played a role to further enhance the CD8 + T cell response where the optimum content was 20–30 mol%. Vaccines giving best protection against solid tumor growth corresponded to the archaeosome adjuvant composition that gave highest immune activity in immunized mice.