Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus. (8th November 2012)

• Record Type: Journal Article
• Authors: Lobasso, Simona; Lopalco, Patrizia; Angelini, Roberto; Vitale, Rita; Huber, Harald; Müller, Volker; Corcelli, Angela
• Other Names: Koga Yosuke Academic Editor.
• Is Part Of: Archaea. Volume 2012(2012)
• Publication Date: 2012-11-08
• Subjects: Archaebacteria -- Periodicals; 579.321
• DOI: 10.1155/2012/957852
• Languages: English
• ISSNs: 1472-3646

Abstract

Abstract : The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N -acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.