Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients. (3rd February 2016)

• Record Type: Journal Article
• Authors: Napoli, Zaleida; Seghieri, Giuseppe; Bianchi, Loria; Anichini, Roberto; De Bellis, Alessandra; Campesi, Ilaria; Carru, Ciriaco; Occhioni, Stefano; Zinellu, Angelo; Franconi, Flavia
• Other Names: Tilton Ronald G. Academic Editor.
• Is Part Of: Journal of diabetes research. Volume 2016(2016)
• Publication Date: 2016-02-03
• Subjects: Diabetes -- Periodicals; Diabetes -- Pathophysiology -- Periodicals; Diabetes -- Prevention -- Periodicals; Diabetes -- Etiology -- Periodicals; Diabetes -- Epidemiology -- Periodicals; Diabetes -- Pathogenesis -- Periodicals; 616.462005
• DOI: 10.1155/2016/7313162
• Languages: English
• ISSNs: 2314-6745

Abstract

Abstract : Background . Taurine transporter gene expression (RNA-TauT) has a role in retinal cell function and is modulated in vitro and in vivo by hyperglycemia and/or oxidative stress. This study was aimed at testing whether RNA-TauT gene expression is modified in blood mononuclear peripheral cells (MPCs) of type 1 diabetic patients, is related to plasma markers of oxidative stress or endothelial dysfunction, or, finally, is related to presence of retinopathy. Methods . RNA-TauT was measured in MPCs by real-time PCR-analysis in 35 type 1 diabetic patients and in 33 age- and sex-matched controls, additionally measuring plasma and cell taurine and markers of oxidative stress and endothelial dysfunction. Results . RNA-TauT, expressed as2 - Δ Δ C t, was significantly higher in MPCs of type 1 diabetic patients than in controls [median (interquartile range): 1.32(0.31) versus 1.00(0.15);P = 0.01 ]. In diabetic patients RNA-TauT was related to HbA1c (r = 0.42 ;P = 0.01 ) and inversely to plasma homocysteine (r = - 0.39 ;P = 0.02 ) being additionally significantly higher in MPCs of patients without retinopathy [(n = 22 ); 1.36(0.34)] compared to those with retinopathy [(n = 13 ); 1.16(0.20)], independently from HbA1c or diabetes duration. Conclusions . RNA-TauT gene expression is significantly upregulated in MPCs of type 1 diabetes patients and is related to HbA1c levels and inversely to plasma homocysteine. Finally, in diabetes patients, RNA-TauT upregulation seems to be blunted in patients with retinopathy independently of their metabolic control or longer diabetes duration.