Axonemal doublet microtubules can split into two complete singlets in human sperm flagellum tips. Issue 9 (19th April 2019)

• Record Type: Journal Article
• Authors: Zabeo, Davide; Croft, Jacob T.; Höög, Johanna L.
• Is Part Of: FEBS letters. Volume 593:Issue 9(2019)
• Page Start: 892
• Page End: 902
• Publication Date: 2019-04-19
• Subjects: axoneme -- cilia -- cryo‐electron microscopy -- cryo‐electron tomography -- fibrous sheath -- singlet region; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13379
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Motile flagella are crucial for human fertility and embryonic development. The distal tip of the flagellum is where growth and intra‐flagellar transport are coordinated. In most model organisms, but not all, the distal tip includes a 'singlet region', where axonemal doublet microtubules (dMT) terminate and only complete A‐tubules extend as singlet microtubules (sMT) to the tip. How a human flagellar tip is structured is unknown. Here, the flagellar tip structure of human spermatozoa was investigated by cryo‐electron tomography, revealing the formation of a complete sMT from both the A‐tubule and B‐tubule of dMTs. This different tip arrangement in human spermatozoa shows the need to investigate human flagella directly in order to understand their role in health and disease. Abstract :