N‐glycome inheritance from cells to extracellular vesicles in B16 melanomas. Issue 9 (11th April 2019)

• Record Type: Journal Article
• Authors: Harada, Yoichiro; Kizuka, Yasuhiko; Tokoro, Yuko; Kondo, Kiyotaka; Yagi, Hirokazu; Kato, Koichi; Inoue, Hiromasa; Taniguchi, Naoyuki; Maruyama, Ikuro
• Is Part Of: FEBS letters. Volume 593:Issue 9(2019)
• Page Start: 942
• Page End: 951
• Publication Date: 2019-04-11
• Subjects: asparagine‐linked glycans -- extracellular vesicles -- metastasis; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13377
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : We investigated the correlation between metastatic behaviors of tumor cells and asparagine‐linked glycosylation (N‐glycosylation) of tumor‐derived extracellular vesicles (EVs). Three mouse melanoma B16 variants with distinct metastatic potentials show similar gene expression levels and enzymatic activities of glycosyltransferases involved in N‐glycosylation. All melanoma variants and EVs have nearly identical profiles of de‐sialylated N‐glycans. The major de‐sialylated N‐glycan structures of cells and EVs are core‐fucosylated, tetra‐antennary N‐glycans with β1, 6‐ N ‐acetylglucosamine branches. A few N‐glycans are extended by N ‐acetyllactosamine repeats. Sialylation of these N‐glycans may generate cell‐type‐specific N‐glycomes on EVs. Taken together, melanoma‐derived EVs show high expression of tumor‐associated N‐glycans, and the core structure profile is inherited during multiple selection cycles of B16 melanomas and from tumor cells to EVs. Abstract : Glycosyltransferase profile was similar between three mouse B16 variants. The three B16 variants and their EVs had nearly identical core N‐glycan profiles. Core‐fucosylated, tetra‐antennary N‐glycans were identified as major structures. N ‐Acetyllactosamine repeats were detected in EVs from all variants. Sialylation may generate cell‐type‐specific N‐glycome on EVs.