Human adenovirus replication and persistence in hypertrophic adenoids and palatine tonsils in children. Issue 7 (18th March 2019)

• Record Type: Journal Article
• Authors: Proenca‐Modena, José Luiz; de Souza Cardoso, Ricardo; Criado, Miriã Ferreira; Milanez, Guilherme Paier; de Souza, William Marciel; Parise, Pierina Lorencini; Bertol, Jéssica Wildgrube; de Jesus, Bruna Lais Santos; Prates, Mirela Cristina Moreira; Silva, Maria Lúcia; Buzatto, Guilherme Pietrucci; Demarco, Ricardo Cassiano; Valera, Fabiana Cardoso Pereira; Tamashiro, Edwin; Anselmo‐Lima, Wilma Terezinha; Arruda, Eurico
• Is Part Of: Journal of medical virology. Volume 91:Issue 7(2019)
• Page Start: 1250
• Page End: 1262
• Publication Date: 2019-03-18
• Subjects: chronic adenotonsillar disease -- hexon mRNA -- human adenovirus (HAdV); Virology -- Periodicals; 616
• DOI: 10.1002/jmv.25441
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract: The role of human adenovirus (HAdV) infection in different acute diseases, such as febrile exudative tonsillitis, conjunctivitis, and pharyngoconjunctival fever is well established. However, the relationships, if any, of HAdV persistence and reactivation in the development of the chronic adenotonsillar disease is not fully understood. The present paper reports a 3‐year cross‐sectional hospital‐based study aimed at detecting and quantifying HAdV DNA and mRNA of the HAdV hexon gene in adenoid and palatine tonsil tissues and nasopharyngeal secretions (NPS) from patients with adenotonsillar hypertrophy or recurrent adenotonsillitis. HAdV C, B, and E were detectable in nearly 50% of the patients, with no association with the severity of airway obstruction, nor with the presence of recurrent tonsillitis, sleep apnea or otitis media with effusion (OME). Despite the higher rates of respiratory viral coinfections in patients with HAdV, the presence of other viruses, including DNA and RNA viruses, had no association with HAdV replication or shedding in secretions. Higher HAdV loads in adenoids showed a significant positive correlation with the presence of sleep apnea and the absence of OME. Although this study indicates that a significant proportion (~85%) of individuals with chronic adenotonsillar diseases have persistent nonproductive HAdV infection, including those by HAdV C, B, and E, epithelial and subepithelial cells in tonsils seem to be critical for HAdV C production and shedding in NPS in some patients, since viral antigen was detected in these regions by immunohistochemistry in four patients, all of which were also positive for HAdV mRNA detection. Highlights: Adenoids and palatine tonsils from patients with chronic adenotonsilar disease are frequently infected with HAdV‐B, HAdV‐C and HAdV‐E. Only a small proportion of patients with the chronic adenotonsilar disease has a productive adenoviral infection, characterized by the detection of viral gene expression. There are no obvious association between adenovirus replication and viral coinfection. HAdV infection was not associated with the severity of nasal obstruction or the presence of recurrent tonsillitis.