Development and optimization of an immunoassay for the detection of Hg(II) in lake water. Issue 5 (6th April 2019) - Jin, Xuexue
Abstract: In this paper, an indirect competitive enzyme‐linked immunosorbent assay (IC‐ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb‐A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween‐20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC50 ) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti‐Hg mAbs also have some affinity with methyl mercury (CH3 Hg) and with no cross‐reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water. Abstract : A direct competitive ELISA for the detection of Hg(II) in tap water and lake water samples has been developed and optimized. ELISA kit components were optimized for better sensitivity and decreasing interference. The IC50 for the developed assay was 1.68 ng/ml. The average recovery rates were between 91% and 116%.