AP-1-Targeting Anti-Inflammatory Activity of the Methanolic Extract of Persicaria chinensis. (23rd March 2015)

• Record Type: Journal Article
• Authors: Hossen, Muhammad Jahangir; Kim, Seung Cheol; Son, Young-Jin; Baek, Kwang-Soo; Kim, Eunji; Yang, Woo Seok; Jeong, Deok; Park, Jae Gwang; Kim, Han Gyung; Chung, Woo-Jae; Yoon, Keejung; Ryou, Chongsuk; Lee, Sang Yeol; Kim, Jong-Hoon; Cho, Jae Youl
• Other Names: Liu Cun-Zhi Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2015(2015)
• Publication Date: 2015-03-23
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2015/608126
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : In traditional Chinese medicine, Persicaria chinensis L. has been prescribed to cure numerous inflammatory disorders. We previously analyzed the bioactivity of the methanol extract of this plant (Pc-ME) against LPS-induced NO and PGE2 in RAW264.7 macrophages and found that it prevented HCl/EtOH-induced gastric ulcers in mice. The purpose of the current study was to explore the molecular mechanism by which Pc-ME inhibits activator protein- (AP-) 1 activation pathway and mediates its hepatoprotective activity. To investigate the putative therapeutic properties of Pc-ME against AP-1-mediated inflammation and hepatotoxicity, lipopolysaccharide- (LPS-) stimulated RAW264.7 and U937 cells, a monocyte-like human cell line, and an LPS/D-galactosamine- (D-GalN-) induced acute hepatitis mouse model were employed. The expression of LPS-induced proinflammatory cytokines including interleukin- (IL-) 1 β, IL-6, and tumor necrosis factor- α (TNF- α ) was significantly diminished by Pc-ME. Moreover, Pc-ME reduced AP-1 activation and mitogen-activated protein kinase (MAPK) phosphorylation in both LPS-stimulated RAW264.7 cells and differentiated U937 cells. Additionally, we highlighted the hepatoprotective and curative effects of Pc-ME pretreated orally in a mouse model of LPS/D-GalN-intoxicated acute liver injury by demonstrating the significant reduction in elevated serum AST and ALT levels and histological damage. Therefore, these results strongly suggest that Pc-ME could function as an antihepatitis remedy suppressing MAPK/AP-1-mediated inflammatory events.