Effect of TongXie-YaoFang on Cl− and HCO3- Transport in Diarrhea-Predominant Irritable Bowel Syndrome Rats. (14th June 2016)

• Record Type: Journal Article
• Authors: Lu, Xiaofang; Zhang, Shengsheng; Yang, Cheng; Wang, Zhengfang; Zhao, Luqing; Wu, Zhenyu; Xie, Jing
• Other Names: Yin Jieyun Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2016(2016)
• Publication Date: 2016-06-14
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2016/7954982
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : TongXie-YaoFang (TXYF) can effectively alleviate the symptoms of diarrhea-predominant irritable bowel syndrome (D-IBS) patients. However, the curative mechanism has not been fully clarified. The study was designed to investigate the effect of TXYF on the colonic ion transport induced by serotonin (5-HT) in D-IBS rats. A method of multiple stress (neonatal maternal separation (NMS) combined with restraint stress (RS)) was used to induce the D-IBS model. The model rats were randomly divided into two groups: NMS + RS group and TXYF-formula group, and the normal control (no handling) rats were classified as NH group. In the NMS + RS group, the change of short-circuit current (Δ I s c ) induced by 5-HT was lower than that in the NH and TXYF-formula groups. After removing of the extracellular Cl − orH C O 3 - or basolateral Na + or blocking the cystic fibrosis transmembrane conductance regulator (CFTR), Na + -K + -2Cl − cotransporter (NKCC), N a + - H C O 3 - cotransporter, C l - / H C O 3 - exchanger, K + channel, or Na + /K + -ATPase, respectively, there was no difference in 5-HT-inducedΔ I s c among the three groups. These data suggest that TXYF can regulate 5-HT-induced Cl − andH C O 3 - secretion, possibly mediated by the combined action of CFTR, NKCC, Na + -H C O 3 - cotransporter, C l - / H C O 3 - exchanger, K + channel, and Na + /K + -ATPase.