Antiobesity Effects of Unripe Rubus coreanus Miquel and Its Constituents: An In Vitro and In Vivo Characterization of the Underlying Mechanism. (19th January 2016)

• Record Type: Journal Article
• Authors: Oh, Dool-Ri; Kim, Yujin; Choi, Eun-jin; Hunmi-Lee, ; Jung, Myung-A; Bae, Donghyuck; Jo, Ara; Kim, Young Ran; Kim, Sunoh
• Other Names: Chan Shun-Wan Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2016(2016)
• Publication Date: 2016-01-19
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2016/4357656
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Background . The objective of the present study was to perform a bioguided fractionation of unripe Rubus coreanus Miquel (uRC) and evaluate the lipid accumulation system involvement in its antiobesity activity as well as study the uRC mechanism of action. Results . After the fractionation, the BuOH fraction of uRC (uRCB) was the most active fraction, suppressing the differentiation of 3T3-L1 adipocytes in a dose-dependent manner. Moreover, after an oral administration for 8 weeks in HFD-induced obese mice, uRCB (10 and 50 mg/kg/day) produced a significant decrease in body weight, food efficiency ratio, adipose tissue weight and LDL-cholesterol, serum glucose, TC, and TG levels. Similarly, uRCB significantly suppressed the elevated mRNA levels of PPAR γ in the adipose tissue in vivo . Next, we investigated the antiobesity effects of ellagic acid, erycibelline, 5-hydroxy-2-pyridinemethanol, m-hydroxyphenylglycine, and 4-hydroxycoumarin isolated from uRCB. Without affecting cell viability, five bioactive compounds decreased the lipid accumulation in the 3T3-L1 cells and the mRNA expression levels of key adipogenic genes such as PPAR γ, C/EBP α, SREBP-1c, ACC, and FAS. Conclusion . These results suggest that uRC and its five bioactive compounds may be a useful therapeutic agent for body weight control by downregulating adipogenesis and lipogenesis.