In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus. (19th December 2010)

• Record Type: Journal Article
• Authors: Miller, Laura C.; Neill, John D.; Harhay, Gregory P.; Lager, Kelly M.; Laegreid, William W.; Kehrli, Marcus E.
• Other Names: Rottier Peter J. M. Academic Editor.
• Is Part Of: Advances in virology. Volume 2010(2010)
• Publication Date: 2010-12-19
• Subjects: Virology -- Periodicals; 616.9101
• DOI: 10.1155/2010/864181
• Languages: English
• ISSNs: 1687-8639

Abstract

Abstract : Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of swine worldwide and causes considerable economic loss. Identifying specific cell signaling or activation pathways that associate with variation in PRRSV replication and macrophage function may lead to identification of novel gene targets for the control of PRRSV infection. Serial Analysis of Gene Expression (SAGE) was used to create and survey the transcriptome of in vitro mock-infected and PRRSV strain VR-2332-infected porcine alveolar macrophages (PAM) at 0, 6, 12, 16, and 24 hours after infection. The transcriptome data indicated changes in transcript abundance occurring in PRRSV-infected PAMs over time after infection with more than 590 unique tags with significantly altered transcript abundance levels identified (P < .01 ). Strikingly, innate immune genes (whose transcript abundances are typically altered in response to other pathogens or insults including IL-8, CCL4, and IL-1 β ) showed no or very little change at any time point following infection.