Antibacterial Activity of Coumarins and Carbazole Alkaloid from Roots of Clausena anisata. (3rd February 2019)

• Record Type: Journal Article
• Authors: Tamene, Dandena; Endale, Milkyas
• Other Names: Oike Masahiro Academic Editor.
• Is Part Of: Advances in pharmacological sciences. Volume 2019(2019)
• Publication Date: 2019-02-03
• Subjects: Pharmacology -- Periodicals; Pharmacological Phenomena; Pharmacology; Periodicals; 615.1
• DOI: 10.1155/2019/5419854
• Languages: English
• ISSNs: 1687-6334

Abstract

Abstract : Clausena anisata is one of the medicinal plants used traditionally for treatment of parasitic infections, irritation (boils, ringworm, and eczema), flatworm infestations, influenza, abdominal cramps, and constipation. Phytochemical screening test of dichloromethane/methanol (1 : 1) roots extract revealed the presence of flavonoids, phytosterols, coumarins, phenols, alkaloids, tannins, terpenoids, and free reducing sugars and the absence of saponins. Silica gel column chromatographic separation of the dichloromethane/methanol (1 : 1) extract afforded a carbazole alkaloid derivative of heptazoline (1 ) and three coumarins (2 –4 ), including the known coumarins imperatorin (3 ) and chalepin (4 ). Structures of the compounds were elucidated by spectroscopic techniques (IR, 1 H NMR, 13 C NMR, and DEPT-135). Antibacterial activity of the crude extracts and isolated compounds was screened using agar diffusion method against strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus substilis . The results of antibacterial test revealed derivative of heptaphylline (1 ) and imperatorin (3 ) exhibited comparable antibacterial activity against S. aureus and B. substilis (14 and 13 mm zone of inhibition, respectively) to that of ciprofloxacin (15 mm zone of inhibition) at a concentration of 20 µ g/mL. Chalepin (4 ) revealed more antibacterial activity against B. substilis (16 mm zone of inhibition) compared to ciprofloxacin (15 mm).