The Immunomodulatory Effects of Albumin In Vitro and In Vivo. (4th April 2011)

• Record Type: Journal Article
• Authors: Wheeler, Derek S.; Giuliano, John S.; Lahni, Patrick M.; Denenberg, Alvin; Wong, Hector R.; Zingarelli, Basilia
• Other Names: Wheeler William J. Academic Editor.
• Is Part Of: Advances in pharmacological sciences. Volume 2011(2011)
• Publication Date: 2011-04-04
• Subjects: Pharmacology -- Periodicals; Pharmacological Phenomena; Pharmacology; Periodicals; 615.1
• DOI: 10.1155/2011/691928
• Languages: English
• ISSNs: 1687-6334

Abstract

Abstract : Albumin appears to have proinflammatory effects in vitro . We hypothesized that albumin would induce a state of tolerance to subsequent administration of lipopolysaccharide (LPS) in vitro and in vivo . RAW264.7 and primary peritoneal macrophages were treated with increasing doses of bovine serum albumin (BSA) and harvested for NF- κ B luciferase reporter assay or TNF- α ELISA. In separate experiments, RAW264.7 cells were preconditioned with 1 mg/mL BSA for 18 h prior to LPS (10 μ g/mL) treatment and harvested for NF- κ B luciferase reporter assay or TNF- α ELISA. Finally, C57Bl/6 mice were preconditioned with albumin via intraperitoneal administration 18 h prior to a lethal dose of LPS (60 mg/kg body wt). Blood was collected at 6 h after LPS administration for TNF- α ELISA. Albumin produced a dose-dependent and TLR-4-dependent increase in NF- κ B activation and TNF- α gene expression in vitro . Albumin preconditioning abrogated the LPS-mediated increase in NF- κ B activation and TNF- α gene expression in vitro and in vivo . The clinical significance of these findings remains to be elucidated.