Development of an Antioxidant Phytoextract of Lantana grisebachii with Lymphoprotective Activity against In Vitro Arsenic Toxicity. (5th June 2014)

• Record Type: Journal Article
• Authors: Soria, Elio A.; Quiroga, Patricia L.; Albrecht, Claudia; Ramos Elizagaray, Sabina I.; Cantero, Juan J.; Bongiovanni, Guillermina A.
• Other Names: Gogal Robert Academic Editor.
• Is Part Of: Advances in pharmacological sciences. Volume 2014(2014)
• Publication Date: 2014-06-05
• Subjects: Pharmacology -- Periodicals; Pharmacological Phenomena; Pharmacology; Periodicals; 615.1
• DOI: 10.1155/2014/416761
• Languages: English
• ISSNs: 1687-6334

Abstract

Abstract : Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-) induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae) (LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T-activated cell culturesP < 0.0001 . Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG-1i at 100 μ g/mL), which protected them against in vitro As-induced lymphotoxicityP < 0.0001 . This infusive LG phytoextract contained10.23 ± 0.43 mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723 mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of ω 9, followed by ω 6 (~24%) and ω 3 (~4%), and scarce ω 7. Summing up, L. grisebachii was a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctions.