Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth. (16th March 2015)

• Record Type: Journal Article
• Authors: Zhu, Hailin; Yang, Jinliang; Wei, Yuquan; Chen, Harry Huimin
• Other Names: Heng Boon Chin Academic Editor.
• Is Part Of: Stem cells international. Volume 2015(2015)
• Publication Date: 2015-03-16
• Subjects: Stem Cells -- Periodicals; Stem Cells -- Therapeutic use -- Periodicals; Stem Cells -- Transplantation -- Periodicals; 616.0277405
• DOI: 10.1155/2015/621057
• Languages: English
• ISSNs: 1687-966X

Abstract

Abstract : Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs. In addition, this newly developed xeno-free substrate can be stored at 4°C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications.