Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. (17th October 2018) - Chaturvedi, Chandra Prakash
Abstract : We have investigated the expression of RNA transcripts of hematopoiesis regulatory molecules, viz., macrophage inflammatory protein (MIP)-1 α, tumor necrosis factor (TNF)- α, granulocyte colony-stimulating factor (G-CSF), stromal cell-derived factor (SDF)-1 α, stem cell factor (SCF), and transforming growth factor (TGF)- β in lipopolysaccharide-induced bone marrow mesenchymal stem cells (BM-MSCs) and levels of their soluble forms in the culture supernatants of BM-MSCs and BM plasma of patients with acquired aplastic anemia (AA) (n = 29 ) and controls (n = 29 ). The BM-MSCs of AA patients as compared to controls had markedly lower expression of MIP-1 α transcripts (p < 0.001 ), higher expression of TNF- α (p < 0.001 ), G-CSF (p < 0.001 ), and SDF-1 α (p < 0.01 ) transcripts, and no difference in the expression of SCF and TGF- β transcripts. The culture supernatants of BM-MSCs and BM plasma of AA patients in comparison to controls also had lower levels of MIP-1 α (p < 0.01 andp < 0.001, respectively) and higher levels of TNF- α (p < 0.05 for both) and G-CSF (p < 0.05 andp < 0.001, respectively) but with no difference in the levels of SDF-1 α and SCF. The levels of TGF- β were although similar in culture supernatants of BM-MSCs of both the groups, but they were significantly lower in BM plasma of the patients than controls (p < 0.001 ). Our data shows that BM-MSCs of AA patients have altered expression of hematopoiesis regulatory molecules suggesting that they may have a role in the pathogenesis of the disease.