Surface Modification of Gelatin Nanoparticles with Polyethylenimine as Gene Vector. (18th April 2011)

• Record Type: Journal Article
• Authors: Kuo, Wei-Ti; Huang, Hong-Yi; Chou, Min-Ju; Wu, Meng-Chao; Huang, Yi-You
• Other Names: Joshi Rakesh Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2011(2011)
• Publication Date: 2011-04-18
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2011/646538
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : A novel carrier on balancing the transfection efficiency and minimizing cytotoxicity was designed. Gelatin cross-linked with 1.8 kDa of PEI (GA-PEI 1.8 k) formed stable complex and resulted in high positive ζ potential (42.47 mV) and buffering effect. These nanoparticles with N/P ratio of 30 give high transfection efficiency2.12 × 10 4 RLU/ μ g protein and cell viability (86.4%). These modified GA-PEI nanoparticles, with high transfection efficiency and low cell toxicity, can be a potential gene vector in gene therapy.