Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line. (21st February 2018)

• Record Type: Journal Article
• Authors: Wu, Xiangju; Qi, Jing; Cong, Xiaoyan; Chen, Lei; Hu, Yue; Yoo, Dongwan; Wang, Guisheng; Tian, Fulin; Li, Feng; Sun, Wenbo; Chen, Zhi; Guo, Lihui; Wu, Jiaqiang; Li, Jun; Wang, Jinbao; Zhao, Xiaomin; Du, Yijun
• Other Names: Zhang Yanjin Academic Editor.
• Is Part Of: BioMed research international. Volume 2018(2018)
• Publication Date: 2018-02-21
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2018/4315861
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.