Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep. (24th April 2012)

• Record Type: Journal Article
• Authors: Pérez de Diego, Ana Cristina; Sánchez-Cordón, Pedro José; de las Heras, Ana Isabel; Sánchez-Vizcaíno, José Manuel
• Other Names: Carrillo Elisa Academic Editor.; Kester Kent E. Academic Editor.
• Is Part Of: TheScientificWorldjournal. Volume 2012(2012)
• Publication Date: 2012-04-24
• Subjects: Science -- Periodicals; Technology -- Periodicals; Medicine -- Periodicals; 505
• DOI: 10.1100/2012/147158
• Languages: English
• ISSNs: 2356-6140

Abstract

Abstract : The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4 +, CD8 +, and WC1 + ), CD25 + regulatory cells, or CD14 + monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14 + monocytes, CD25 + regulatory cells, and CD8 + cytotoxic T lymphocytes.