Phenotypic Definition of the Progenitor Cells with Erythroid Differentiation Potential Present in Human Adult Blood. (26th September 2011)

• Record Type: Journal Article
• Authors: Tirelli, Valentina; Ghinassi, Barbara; Migliaccio, Anna Rita; Whitsett, Carolyn; Masiello, Francesca; Sanchez, Massimo; Migliaccio, Giovanni
• Other Names: Sadelain Michel Academic Editor.
• Is Part Of: Stem cells international. Volume 2011(2011)
• Publication Date: 2011-09-26
• Subjects: Stem Cells -- Periodicals; Stem Cells -- Therapeutic use -- Periodicals; Stem Cells -- Transplantation -- Periodicals; 616.0277405
• DOI: 10.4061/2011/602483
• Languages: English
• ISSNs: 1687-966X

Abstract

Abstract : In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid cells (EBs) than the correspondingC D 34 p o s cells, suggesting that MNC may also containC D 34 n e g HPC. To clarify the phenotype of AB HPC which generate EBs in these cultures, flow cytometric profiling for CD34/CD36 expression, followed by isolation and functional characterization (colony-forming-ability in semisolid-media and fold-increase in HEMA) were performed. Four populations with erythroid differentiation potential were identified:C D 34 p o s C D 36 n e g (0.1%);C D 34 p o s C D 36 p o s (barely detectable-0.1%);C D 34 n e g C D 36 l o w (2%) andC D 34 n e g C D 36 n e g (75%). In semisolid-media, C D 34 p o s C D 36 n e g cells generated BFU-E and CFU-GM (in a 1 : 1 ratio), C D 34 n e g C D 36 n e g cells mostly BFU-E (87%) andC D 34 p o s C D 36 p o s andC D 34 n e g C D 36 l o w cells were not tested due to low numbers. Under HEMA conditions, C D 34 p o s C D 36 n e g, C D 34 p o s C D 36 p o s, C D 34 n e g C D 36 l o w andC D 34 n e g C D 36 n e g cells generated EBs with fold-increases of ≈ 9, 000, 100, 60 and 1, respectively, and maturation times (day with >10%C D 36 h i g h C D 235 a h i g h cells) of 10–7 days. Pyrenocytes were generated only byC D 34 n e g /C D 36 n e g cells by day 15. These results confirm that the majority of HPC in AB express CD34 but identify additionalC D 34 n e g populations with erythroid differentiation potential which, based on differences in fold-increase and maturation times, may represent a hierarchy of HPC present in AB.