Flow Cytometric Evaluation of T Cell Activation Markers after Cardiopulmonary Bypass. (6th February 2014)

• Record Type: Journal Article
• Authors: Dieterlen, Maja-Theresa; Bittner, Hartmuth B.; Tarnok, Attila; Garbade, Jens; Dhein, Stefan; Mohr, Friedrich W.; Barten, Markus J.
• Other Names: Strecker Thomas Academic Editor.
• Is Part Of: Surgery research and practice. Volume 2014(2014)
• Publication Date: 2014-02-06
• Subjects: Surgery -- Periodicals; 617.005
• DOI: 10.1155/2014/801643
• Languages: English
• ISSNs: 2356-7759

Abstract

Abstract : Background . Cardiopulmonary bypass surgery (CPBS) is associated with an increased risk for infections or with subsequent organ dysfunction. As T cell activation is a central mechanism during inflammatory processes, we developed an assay to evaluate T cell activation pathways in patients undergoing CPBS. Methods . Blood was obtained from eleven patients undergoing CPBS preoperatively, on postoperative day (POD)-3, and on POD-7 and was stimulated with different concentrations of Concanavalin A (ConA). Cyclosporine and sirolimus, inhibiting different pathways of the T cell cycle, were added to blood ex vivo. Expression of T cell activation markers CD25 and CD95 was analyzed by flow cytometry. Results . In untreated blood, expression of CD25 and CD95 significantly increased with higher ConA concentrations( P < 0.05 ) and decreased for all ConA concentrations for both antigens over the study time( P < 0.05 ) . Independently from the ConA concentration, inhibition of CD25 and CD95 expression was highest preoperatively for sirolimus and on POD-3 for cyclosporine. At all time points, inhibition of CD25 and CD95 expression was significantly higher after cyclosporine compared to sirolimus treatment( P < 0.001 ) . Conclusion . Our results showed that different pathways of T cell activation are impaired after CPBS. Such knowledge may offer the opportunity to identify patients at risk for postoperative complications.