Adoptive Transfers of CD4+CD25+ Tregs Raise Foxp3 Expression and Alleviate Mouse Enteritis. (30th September 2018)

• Record Type: Journal Article
• Authors: Wang, Kai; Zhu, Tongjia; Wang, Haijun; Yang, Jinxin; Du, Shuaishuai; Dong, Guoying; Pei, Zhihua; Hu, Guixue
• Other Names: Palomares Oscar Academic Editor.
• Is Part Of: BioMed research international. Volume 2018(2018)
• Publication Date: 2018-09-30
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2018/9064073
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : CD4 + CD25 + Foxp3 + Tregs control the immune response and maintain immune homeostasis. This study examined whether Tregs can affect mouse enteritis and the Foxp3 (Forkhead transcription factor) transcriptional pathway. Mouse CD4 + CD25 + Treg cells were labelled using CFSE (5, 6-carboxyfluorescein diacetate succinimidyl ester) and transferred to enteritis model mice. The mice were randomly divided into an enteritis group, a Treg-infusion group, a Treg-inhibiting group, and a control group. Histopathology, ELISA, flow cytometry, western blot, immunohistochemistry, and immunofluorescence were performed. Our results demonstrated that CD4 + CD25 + Tregs were successfully transferred. The disease activity index (DAI) scores in the Tregs-infusion group were lower than those of the enteritis and Tregs-inhibiting groups. The number of goblet cells and inflammatory cells was reduced, and the levels of IL-1 β, TNF- α, NO, and PGE2 were significantly decreased in the Tregs-infusion group compared to those in the enteritis group (p<0.05). The number of CD4 + CD25 + Foxp3 + Tregs and CD4 + IL-17A + Th17 cells in the mesenteric lymph nodes differed significantly from the enteritis and Tregs-inhibiting groups (p<0.05). There were more Foxp3 + Tregs and Smad3 and NFAT2 infiltrated into the duodenum after adoptive transfer of CD4 + CD25 + Tregs, which was a significant difference relative to the enteritis group (p<0.05). This study demonstrated that adoptive transfer of CD4 + CD25 + Tregs can decrease mouse enteritis. Foxp3 expression may be improved through the Smad3 and NFAT2 signalling pathways.