Nanoparticle-Mediated Photodynamic Therapy for Mixed Biofilms. (16th August 2016)

• Record Type: Journal Article
• Authors: Usacheva, Marina; Layek, Buddhadev; Rahman Nirzhor, Saif S.; Prabha, Swayam
• Other Names: Ding Jianxun Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2016(2016)
• Publication Date: 2016-08-16
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2016/4752894
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : Wounds associated with diabetes are difficult to heal and often stay unhealed, leading to higher morbidity and mortality in the diabetic population. A major barrier to the successful healing of chronic diabetic wounds is the presence of biofilm-mediated infections. In recent years, photodynamic therapy (PDT) has emerged as a promising treatment modality for the management of chronic wounds. The objective of the present study was to evaluate the effectiveness of toluidine blue- (TB-) loaded nanoparticle-mediated PDT in eradicating antibiotic-resistant polymicrobial biofilms of Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus in an in vitro model. Dioctyl sodium sulfosuccinate (aerosol OT, AOT)-alginate nanoparticles with high TB loading (10.8 ± 2.2 %) were formulated using a double emulsification cross-linking method. TB nanoparticles induced effective killing of planktonic P . aeruginosa (3.5 log10 CFU) and S . aureus (>5 log10 CFU) and their combined biofilms (2.8 log10 CFU for P . aeruginosa versus 3.4 log10 CFU for S . aureus ). While P . aeruginosa biofilm was more resistant when compared to that of S . aureus, our results demonstrated effective eradication of complex biofilms of dual bacterial strains in vitro .