The Efficacy of Umbelliferone, Arbutin, and N-Acetylcysteine to Prevent Microbial Colonization and Biofilm Development on Urinary Catheter Surface: Results from a Preliminary Study. (5th April 2016)

• Record Type: Journal Article
• Authors: Cai, Tommaso; Gallelli, Luca; Meacci, Francesca; Brugnolli, Anna; Prosperi, Letizia; Roberta, Stefani; Eccher, Cristina; Mazzoli, Sandra; Lanzafame, Paolo; Caciagli, Patrizio; Malossini, Gianni; Bartoletti, Riccardo
• Other Names: Vanittanakom Nongnuch Academic Editor.
• Is Part Of: Journal of pathogens. Volume 2016(2016)
• Publication Date: 2016-04-05
• Subjects: Pathogenic microorganisms -- Periodicals; Bacteria -- pathogenicity; Microbiological Phenomena; Infection; Pathogenic microorganisms; Electronic journals; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; 616.9
• DOI: 10.1155/2016/1590952
• Languages: English
• ISSNs: 2090-3057

Abstract

Abstract : We evaluated, in a preliminary study, the efficacy of umbelliferone, arbutin, and N-acetylcysteine to inhibit biofilm formation on urinary catheter. We used 20 urinary catheters: 5 catheters were incubated with Enterococcus faecalis (control group); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 1); 5 catheters were incubated with E. faecalis in presence of umbelliferone (150 mg), arbutin (60 mg), and N-acetylcysteine (400 mg) (group 2); and 5 catheters were incubated with E. faecalis in presence of umbelliferone (300 mg), arbutin (60 mg), and N-acetylcysteine (150 mg) (group 3). After 72 hours, planktonic microbial growth and microorganisms on catheter surface were assessed. In the control group, we found a planktonic load of ≥10 5 CFU/mL in the inoculation medium and retrieved 3.69 × 10 6 CFU/cm from the sessile cells adherent to the catheter surface. A significantly lower amount in planktonic (p < 0.001 ) and sessile (p = 0.004 ) bacterial load was found in group 3, showing <100 CFU/mL and 0.12 × 10 6 CFU/cm in the incubation medium and on the catheter surface, respectively. In groups 1 and 2, 1.67 × 10 6 CFU/cm and 1.77 × 10 6 CFU/cm were found on catheter surface. Our results document that umbelliferone, arbutin, and N-acetylcysteine are able to reduce E. faecalis biofilm development on the surface of urinary catheters.