The KM-Algorithm Identifies Regulated Genes in Time Series Expression Data. (7th October 2009)

• Record Type: Journal Article
• Authors: Bremer, Martina; Doerge, R. W.
• Other Names: Zhao Zhongming Academic Editor.
• Is Part Of: Advances in bioinformatics. Volume 2009(2009)
• Publication Date: 2009-10-07
• Subjects: Bioinformatics -- Periodicals; Bioinformatics; Computational Biology -- Periodicals; Periodicals; 570.285
• DOI: 10.1155/2009/284251
• Languages: English
• ISSNs: 1687-8027

Abstract

Abstract : We present a statistical method to rank observed genes in gene expression time series experiments according to their degree of regulation in a biological process. The ranking may be used to focus on specific genes or to select meaningful subsets of genes from which gene regulatory networks can be built. Our approach is based on a state space model that incorporates hidden regulators of gene expression. Kalman (K) smoothing and maximum (M) likelihood estimation techniques are used to derive optimal estimates of the model parameters upon which a proposed regulation criterion is based. The statistical power of the proposed algorithm is investigated, and a real data set is analyzed for the purpose of identifying regulated genes in time dependent gene expression data. This statistical approach supports the concept that meaningful biological conclusions can be drawn from gene expression time series experiments by focusing on strong regulation rather than large expression values.